INTENDED USE For In Vitro Diagnostic Use Invitrogen’s monoclonal Mouse anti-Multidrug Resistance-associated Protein antibody is intended to qualitatively detect Multi-Drug resistant-associated protein in frozen and formalin-fixed, paraffin-embedded tissue sections. Interpretation must be made within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist. BACKGROUND The Multidrug Resistance-Associated Protein (MRP) molecule is an integral membrane glycophosphoprotein belonging to the same superfamily of ATP-binding cassette transmembrance transporter proteins as P-glycoprotein1 . MRP is overexpressed in many P-glycoprotein-negative, multidrug resistant cell lines and tumors2 . The drug resistance activity of MRP may be due to its function as a plasma membrane drug efflux pump3 . Mouse anti-Multidrug Resistance-Associated Protein (Clone: QCRL-1) reacts with a linear epitope on Multidrug Resistance-Associated Protein, a 190 kDa transmembrane phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines4 . REAGENT PROVIDED Mouse anti-Multidrug Resistance-associated Protein Antibody is purified from mouse ascites and diluted in phosphate buffered saline (PBS), pH 7.4, and 1% bovine serum albumin (BSA) with 0.1% sodium azide (NaN3) as a preservative. Immunogen: Membranes prepared from SCLC cell line H69AR Total protein concentration: ??? g/L Clone: QCRL-1 Isotype: IgG1 Antibody concentration: ??? mg/L STORAGE: 2-8°C POSITIVE CONTROL TISSUE: Lung cancer EXPECTED STAINING PATTERN: Cytoplasmic and membrane INSTRUCTIONS FOR USE PRETREATMENT REQUIREMENTS: Epitope Retrieval: Required (Citrate Buffer pH 6.0) (See page 2 for protocol) Enzyme Digestion: Not Required Mouse anti-Multidrug Resistance-associated Protein may be diluted according to Table 1 when using the Invitrogen detection systems below. Table 1. Dilution Table Invitrogen Kit Predilute Antibody Dilution for Concentrate Incubation Time Histostain-SP or SAP kits* Ready-To-Use 1: 50 - 1: 100 60 min. Histostain®-Plus Kits Ready-To-Use 1: 100 - 1: 200 30-60 min. Non-Biotin Amplification (NBA)™ Kits Ready-To-Use 1: 100 - 1: 200 30-60 min. PicTure™-Plus Polymer Kits Ready-To-Use 1: 100 - 1: 200 30-60 min. Cap-Plus™ Kits Ready-To-Use 1: 100 - 1: 200 30-60 min. * Use Histostain-SP or SAP kits only for Cat. No. 08-0XXX and 18-X001 to 18-X200 primary antibodies. This is a guideline only. Optimal antibody concentrations may vary based on specimen and preparation method used, and should be determined by each individual laboratory.